Tag: M.W:300-400

Ahn, Woo-Chan; Aroli, Shashanka; Kim, Jin-Hahn; Moon, Jeong Hee; Lee, Ga Seal; Lee, Min-Ho; Sang, Pau Biak; Oh, Byung-Ha; Varshney, Umesh; Woo, Eui-Jeon published the artcile< Covalent binding of uracil DNA glycosylase UdgX to abasic DNA upon uracil excision>, Safety of ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate, the main research area is covalent binding uracil DNA glycosylase udgx abasic upon excision.

Uracil DNA glycosylases (UDGs) are important DNA repair enzymes that excise uracil from DNA, yielding an abasic site. Recently, UdgX, an unconventional UDG with extremely tight binding to DNA containing uracil, was discovered. The structure of UdgX from Mycobacterium smegmatis in complex with DNA shows an overall similarity to that of family 4 UDGs except for a protruding loop at the entrance of the uracil-binding pocket. Surprisingly, H109 in the loop was found to make a covalent bond to the abasic site to form a stable intermediate, while the excised uracil remained in the pocket of the active site. H109 functions as a nucleophile to attack the oxocarbenium ion, substituting for the catalytic water mol. found in other UDGs. To our knowledge, this change from a catalytic water attack to a direct nucleophilic attack by the histidine residue is unprecedented. UdgX utilizes a unique mechanism of protecting cytotoxic abasic sites from exposure to the cellular environment.

Nature Chemical Biology published new progress about Enzyme functional sites, active. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Safety of ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Hu, Si; Feng, Xi; Huang, Wen; Ibrahim, Salam A.; Liu, Ying published the artcile< Effects of drying methods on non-volatile taste components of Stropharia rugoso-annulata mushrooms.>, Recommanded Product: ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate, the main research area is Stropharia non volatile compound hot air vacuum freeze drying.

In this study, the pileus and stipe of Stropharia rugoso-annulata were sep. dehydrated using natural air drying (NAD), hot air drying (HAD) and vacuum freeze drying (VFD), the proximate composition and non-volatile compounds (soluble sugars, organic acids, free amino acids and 5′-nucleotides) were investigated. We found that the contents of free amino acids (37.21-51.07 mg/g) and 5′-nucleotides (18.33-22.76 mg/g) in pileus were higher than those of stipe. The contents of MSG-like amino acids (3.61-11.70 mg/g) and flavor 5′-nucleotides (5.47-5.94 mg/g) in pileus were also higher than those of stipe. The dried S. rugoso-annulata treated with VFD had the highest levels of soluble sugars, organic acids, free amino acids and 5′-nucleotides. We found that NAD and HAD had medium levels of MSG-like amino acids (7.95-11.7 mg/g) and VFD had low levels of MSG-like amino acids (3.16-3.61 mg/g). Moreover, HAD had the highest level of equivalent umami concentration (EUC) value (784.05 g MSG/100 g). Our results suggested that VFD was better to preserve the non-volatile compounds in S. rugoso-annulata, but HAD would be a potential method to produce umami concentration of dried S. rugoso-annulata.

LWT–Food Science and Technology published new progress about Amino acids Role: FFD (Food or Feed Use), BIOL (Biological Study), USES (Uses). 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Recommanded Product: ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Seifert, Roland; Schirmer, Bastian published the artcile< cCMP and cUMP come into the spotlight, finally>, Reference of 58-97-9, the main research area is cytidylyl uridylyl cyclase identification; base-specificity; cCMP; cUMP; cytidylyl cyclase; second messenger; uridylyl cyclase.

cCMP and cUMP have been identified in numerous biol. systems and proposed to serve as second messengers. However, this proposal remained controversial because of the base-promiscuity of generators, effectors, phosphodiesterases, and bacterial toxins. With the identification of specific cytidylyl and uridylyl cyclases, cCMP and cUMP research enters a new era.

Trends in Biochemical Sciences published new progress about Enzymes Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (Uridylyl cyclase). 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Reference of 58-97-9.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Feliu, Catherine; Peyret, Helene; Vautier, Damien; Djerada, Zoubir published the artcile< Simultaneous quantification of 8 nucleotides and adenosine in cells and their medium using UHPLC-HRMS>, Recommanded Product: ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate, the main research area is nucleotide adenosine UHPLC HRMS; Adenosine; Extracellular; High-resolution mass spectrometry; Intracellular; Nucleotides; Quantification.

Purinergic signaling is involved in physiol. processes, particularly during ischemia-reperfusion injuries for which it has a protective effect. The purpose of this work was to develop a method for simultaneous quantification of eight nucleotides and adenosine in biol. matrixes by liquid chromatog. coupled with high-resolution mass spectrometry. A method was developed that was sufficiently robust to quantify the targeted analytes in 20 min with good sensitivity. Anal. of extracellular media from cultured endothelial cells detected the release of nucleotides and adenosine during 2 h of hypoxia. The quantification of cylic adenosine monophosphate (cAMP) allowed to establish a dose-response curve after receptor stimulation. Therefore, the authors′ method allows the authors to study the involvement of nucleotides in various processes in both the intracellular and extracellular compartment.

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences published new progress about Cardiomyocyte. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Recommanded Product: ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Vlasova, Nataliya; Markitan, Olga published the artcile< Phosphate-nucleotide-nucleic acid: Adsorption onto nanocrystalline ceria surface>, Computed Properties of 58-97-9, the main research area is cerium dioxide deoxyribonucleic acid phosphate nucleotide.

The adsorption of DNA (DNA) and its constituents – phosphate and nucleotides on the surface of nanocrystalline cerium dioxide (pHpzc = 6.3) in NaCl solutions was investigated using multi batch adsorption experiments over a wide range pH. The adsorption data of inorganic phosphate, and nucleotides were interpreted as a formation of outer and inner sphere surface complexes in term of the Basic Stern surface complexation model. The comparison of adsorptions of DNA, phosphate and nucleotides has revealed that double-stranded DNA is mainly adsorbed with the participation of phosphate backbone of its mol. The approach of DNA to the oxide surface due to the electrostatic attraction promotes other types of interaction, e.g. dispersion interaction and hydrogen bonding.

Colloids and Surfaces, A: Physicochemical and Engineering Aspects published new progress about DNA Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Computed Properties of 58-97-9.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Zalawadiya, Sandip K.; Lindenfeld, JoAnn; Shah, Ashish; Wigger, Mark; Danter, Matthew; Brinkley, D. Marshall; Menachem, Jonathan; Punnoose, Lynn; Balsara, Keki; Brown Sacks, Suzanne; Ooi, Henry; Perri, Roman; Awad, Joseph; Smith, Sarah; Fowler, Rachel; O’Dell, Heather; Darragh, Callie; Ruzevich-Scholl, Shelly; Schlendorf, Kelly published the artcile< Trends in Renal Function Among Heart Transplant Recipients of Donor-Derived Hepatitis C Virus>, Application In Synthesis of 58-97-9, the main research area is hepatitis C virus renal impairment heart transplantation sofosbuvir antiviral.

Donor-derived hepatitis C (dd-HCV) infection may increase the risk of renal impairment (RI) among heart transplantation (HT) recipients. Sofosbuvir, an integral component of HCV direct-acting antivirals (DAAs) has also been linked to RI. To date, no study has examined the trends in renal function for HT recipients of dd-HCV infection and assessed safety and efficacy of Sofosbuvir-based DAAs. Between Sept. 2016 and June 2018, 46 HCV-naive patients and one patient with a history of HCV treated pretransplant, underwent HT from HCV-pos. donors (follow-up available through Oct. 10, 2018). Patients were treated with Ledipasvir-Sofosbuvir (genotype 1) or Sofosbuvir-Velpatasvir (genotype 3) for 12 or 24 wk; no dose adjustments were made for renal function. Data on renal function were available for 23 patients who achieved a sustained virol. response at 12 wk after the treatment (SVR12; cohort A) and 18 patients who completed 1 yr of follow-up (cohort B). All patients tolerated DAAs well with 100% completion rate to the assigned therapy and duration and 100% success at achieving SVR12. In this first and largest reported case series to date of HT recipients with dd-HCV infection, we observed that neither the dd-HCV infection nor its treatment with Sofosbuvir-based DAAs increased the risk of RI. Sofosbuvir-based DAAs appear safe, tolerable, and effective for HCV treatment even in presence of severe RI.

ASAIO Journal published new progress about Antiviral agents (direct-acting antiviral). 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Application In Synthesis of 58-97-9.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Zhou, Dong-Dong; Zhang, Hao; Zhang, Qian; Qian, Zheng-Ming; Li, Wen-Jia; Li, Chun-Hong; Yang, Feng-Qing; Chen, Hua published the artcile< Preparation of titanium ion functionalized polydopamine coated ferroferric oxide core-shell magnetic particles for selective extraction of nucleotides from Cordyceps and Lentinus edodes>, Safety of ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate, the main research area is titanium polydopamine ferroferric oxide magnetic particle nucleotide Cordyceps Lentinus; Cordyceps; Lentinus edodes; Magnetic solid phase extraction; Nucleotides; Selective extraction.

In this study, a titanium ion (Ti4+) functionalized polydopamine coated ferroferric oxide (Fe3O4@PDA@Ti4+) core-shell magnetic particle was prepared for the selective extraction of nucleotides. Firstly, different metal ions including Ti4+, Zr4+, Fe3+, Al3+, Cu2+, Zn2+, Ni2+ and Mg2+ were resp. immobilized onto Fe3O4@PDA particles and their extraction efficiency for five nucleotides [cytidine-5′-monophosphate (CMP), uridine-5′-monophosphate (UMP), guanosine-5′-monophosphate (GMP), thymidine-5′-monophosphate (TMP) and adenosine-5′-monophosphate (AMP)] were compared. Among these prepared materials, Fe3O4@PDA@Ti4+, which exhibited the highest extraction efficiency for nucleotides, was further characterized by Fourier transform IR spectroscopy, SEM, TEM and energy dispersive x-ray spectroscopy. After being optimized of the extraction parameters including adsorbent amounts, extraction time, extraction temperature, type and concentration of the eluent, the prepared Fe3O4@PDA@Ti4+ magnetic particles were successfully applied for the selective extraction and determination of CMP, UMP, GMP, TMP and AMP in Cordyceps and Lentinus edodes. Good linearity (varying from 0.063 to 19.000 μg/mL, R2 > 0.999) and low limit of detection (LODs) (ranging between 0.0047 and 0.0141 μg/mL) for target analytes were achieved. These results demonstrated that the synthesized material in this study had potential for selective extraction of phosphorylated small mol. compounds in complicated matrix.

Journal of Chromatography A published new progress about Cordyceps. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Safety of ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Rymen, Daisy; Lindhout, Martijn; Spanou, Maria; Ashrafzadeh, Farah; Benkel, Ira; Betzler, Cornelia; Coubes, Christine; Hartmann, Hans; Kaplan, Julie D.; Ballhausen, Diana; Koch, Johannes; Lotte, Jan; Mohammadi, Mohammad Hasan; Rohrbach, Marianne; Dinopoulos, Argirios; Wermuth, Marieke; Willis, Daniel; Brugger, Karin; Wevers, Ron A.; Boltshauser, Eugen; Bierau, Jorgen; Mayr, Johannes A.; Wortmann, Saskia B. published the artcile< Expanding the clinical and genetic spectrum of CAD deficiency: an epileptic encephalopathy treatable with uridine supplementation>, Formula: C9H13N2O9P, the main research area is anemia epilepticus dyserythropoietic infantile encephalopathy; EIEE; anemia; developmental delay; early infantile epileptic encephalopathy-50; epilepsy.

Abstract: Purpose: Biallelic CAD variants underlie CAD deficiency (or early infantile epileptic encephalopathy-50, [EIEE-50]), an error of pyrimidine de novo biosynthesis amenable to treatment via the uridine salvage pathway. We further define the genotype and phenotype with a focus on treatment. Methods: Retrospective case series of 20 patients. Results: Our study confirms CAD defici. as a progressive EIEE with recurrent status epilepticus, loss of skills, and dyserythropoietic anemia. We further refine the phenotype by reporting a movement disorder as a frequent feature, and add that milder courses with isolated developn. delay/intellectual disability can occur as well as onset with neonatal seizures. With no biomarker availa., the diagnosis relies on genetic testing and functional validation in patient-derived fibroblasts. Underlying pathogenic variants are often rated as variants of unknown significance, which could lead to underrecognition of this treatable disorder. Supplemen. with uridine, uridine monophosphate, or uridine triacetate in 10 patients was safe and led to significant clin. improvn. in most patients. Conclusion: We advise a trial with uridine in all patients with developmental delay/intellectual disability, epilepsy, and anemia; all patients with status epilepticus; and all patients with neonatal seizures until (genetically) proven otherwise or proven unsuccessful after 6 mo. CAD defici. might represent a condition for genetic newborn screening.

Genetics in Medicine published new progress about Anemia. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Formula: C9H13N2O9P.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Shen, Su; Zhang, Xing; Li, Zhimin published the artcile< Development of an engineered carbamoyl phosphate synthetase with released sensitivity to feedback inhibition by site-directed mutation and casting error-prone PCR>, Product Details of C9H13N2O9P, the main research area is Carbamoyl phosphate synthase; Feedback inhibition; Intermolecular interaction; Molecular docking; Protein engineering.

Carbamoyl phosphate synthetase (CPS) is a key enzyme in both pyrimidine and arginine biosynthesis. However, it is inhibited strongly by uridine monophosphate (UMP), which is an intermediate of the de-novo synthesis of pyrimidine nucleoside. In this study, the native carbamoyl phosphate synthetase, from Escherichia coli, was evolved by site-directed mutation and casting error-prone PCR. Compared with the wild-type, the variant N1015 F had released sensitivity to UMP and exhibited 100% of the initial activity in the presence of UMP. Variant K1006A exhibited 0.14-fold improvement in initial activity and kept above 65% of relative activity under the saturated concentration of inhibitor. Structure anal. of variants demonstrated that the reduced sensitivity to inhibitor was largely attributed to the decreased hydrogen bonds, which could reduce the binding affinity with UMP. Also, Phe with large side chain could narrow the binding pocket and generate more steric hindrance. Based on the results in this study, N1015F was an ideal alternative catalyst for the wild-type CPS for pyrimidine biosynthesis.

Enzyme and Microbial Technology published new progress about 58-97-9. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Product Details of C9H13N2O9P.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

de Castro, Sonia; Ferrer-Orta, Cristina; Mills, Alberto; Fernandez-Cureses, Gloria; Gago, Federico; Verdaguer, Nuria; Camarasa, Maria-Jose published the artcile< (F)uridylylated peptides linked to VPg1 of foot-and-mouth disease virus (FMDV): design, synthesis and X-ray crystallography of the complexes with FMDV RNA-dependent RNA polymerase>, Category: tetrahydrofurans, the main research area is furidylylated peptide complex VPg FMDV RNA Xray crystallog; 5-fluorouracil; RNA-dependent RNA polymerase; VPg; foot-and-mouth disease virus; uridylylation inhibition.

Foot-and-mouth disease virus (FMDV) is an RNA virus belonging to the Picornaviridae family that contains three small viral proteins (VPgs), named VPg1, VPg2 and VPg3, linked to the 5′-end of the viral genome. These VPg proteins act as primers for RNA replication, which is initiated by the consecutive binding of two UMP mols. to the hydroxyl group of Tyr3 in VPg. This process, termed uridylylation, is catalyzed by the viral RNA-dependent RNA polymerase named 3Dpol. 5-Fluorouridine triphosphate (FUTP) is a potent competitive inhibitor of VPg uridylylation. Peptide anal. showed FUMP covalently linked to the Tyr3 of VPg. This fluorouridylylation prevents further incorporation of the second UMP residue. The mol. basis of how the incorporated FUMP blocks the incorporation of the second UMP is still unknown. To investigate the mechanism of inhibition of VPg uridylylation by FUMP, we have prepared a simplified 15-mer model of VPg1 containing FUMP and studied its x-ray crystal structure in complex with 3Dpol. Unfortunately, the fluorouridylylated VPg1 was disordered and not visible in the electron d. maps; however, the structure of 3Dpol in the presence of VPg1-FUMP showed an 8 Å movement of the β9-α11 loop of the polymerase towards the active site cavity relative to the complex of 3Dpol with VPg1-UMP. The conformational rearrangement of this loop preceding the 3Dpol B motif seems to block the access of the template nucleotide to the catalytic cavity. This result may be useful in the design of new antivirals against not only FMDV but also other picornaviruses, since all members of this family require the uridylylation of their VPg proteins to initiate the viral RNA synthesis.

Molecules published new progress about Antiviral agents. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Category: tetrahydrofurans.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem