Category: 58-97-9

Dai, Kun; Peng, Xiaoqiang; Zhuang, Wei; Yang, Pengpeng; Jiao, Pengfei; Wu, Jinglan; Ying, Hanjie published the artcile< Mass transfer process and separation mechanism of four 5′-ribonucleotides on a strong acid cation exchange resin>, Name: ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate, the main research area is 5′-ribonucleotides; Homogeneous surface diffusion model; Ion exchange chromatography; Mass transfer process; Physical adsorption; Separation mechanism.

5′-ribonucleotides including AMP (AMP), cytidine 5′-monophsphate (CMP), GMP (GMP) and UMP (UMP) have been widely used in the food and pharmaceutical industries. This work focused on the assessment of mass transfer process and separation mechanism of four 5′-ribonucleotides and counter-ion Na+ on the strong cation exchange resin NH-1. The intraparticle diffusion was determined as the rate-limiting step for the mass transfer of AMP, CMP, GMP, and Na+ on the resin NH-1 through the Boyd model. Meanwhile, a homogeneous surface diffusion model (HSDM) combing ion exchange and phys. adsorption was proposed and tested against adsorption kinetic data in the batch adsorption systems. The fixed-bed film-surface diffusion model based on the HSDM was then developed and successfully predicted the concentration profiles of 5′-ribonucleotides and the change of pH at the outlet of the fixed-bed in the dynamic adsorption and separation process. Finally, the separation mechanism of 5′-ribonucleotides was presented combining model prediction and exptl. results. The separation of UMP, GMP and CMP were mainly based on their differences in isoelec. points, while that of AMP and CMP were lied with the discrepancy of their phys. adsorption binding capacity with the resin NH-1.

Journal of Chromatography A published new progress about 58-97-9. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Name: ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

El-Araby, Hanaa A; Behairy, Behairy E; El-Guindi, Mohamed A; Adawy, Nermin M; Allam, Alif A; Sira, Ahmad M; Khedr, Mohamed A; Elhenawy, Ibrahim A; Sobhy, Gihan A; Basiouny, Hosam El Din M; Salem, Menan E; Abdel-Aziz, Samira A; Fouad, Ola A; Ayoub, Bassam A published the artcile< Generic sofosbuvir/ledipasvir for the treatment of genotype 4 chronic hepatitis C in Egyptian children (9-12 years) and adolescents.>, Recommanded Product: ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate, the main research area is Children; Hepatitis C virus; Ledipasvir; Sofosbuvir.

BACKGROUND/PURPOSE OF THE STUDY: Worldwide and national efforts are directed against eradication of HCV. The introduction of direct-acting antivirals (DAAs) has changed dramatically the outcome of HCV treatment. In spite of the Food and Drug Administration approval of the oral drugs sofosbuvir (SOF) and ledipasvir (LED) for the treatment of HCV in adolescents more than or equal to 12 years old, sufficient real-world experience is still lacking. The aim of this study was to assess the safety and efficacy of the generic SOF/LED fixed-dose combination 400/90 (400 mg SOF + 90 mg LED) for the treatment of adolescents and children (9-12 years) with chronic hepatitis C (CHC). METHODS: In this prospective observational study, 100 cases of genotype 4 CHC were recruited consecutively from those fulfilling the inclusion and exclusion criteria. All cases received the generic fixed-dose combination SOF/LED (400/90), one tablet daily for 12 weeks. All clinical, laboratory, and virologic characteristics were evaluated at base line, and week (W) 2, 4, 8, and 12 of therapy and W12 post-treatment (SVR12). RESULTS: Recruited children (9-12) and adolescents weighed 28-83 and 31-90 kg, respectively. Eighty cases were naïve and 20 cases were pegylated interferon/ribavirin treatment-experienced. Very rapid virologic response (vRVR) at W2 was 96%, while at W4 response rate was 100% and maintained till the end of treatment and at W12 post-treatment (SVR12). All reported side effects were mild and did not lead to treatment termination and disappeared at W12 post-treatment. CONCLUSION: The generic SOF/LED fixed-dose combination is safe and effective in children, 9-12 years, and adolescents with vRVR rate of 96%, 100% EOT response and SVR12.

Hepatology international published new progress about 58-97-9. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Recommanded Product: ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Elbedewy, Tamer A; Abd-Elsalam, Sherief; Mostafa, Sahar Mohamed; Abdellatif, Raghda Samir; Fouad, Amina; Youssef, Mona; Abo-Amer, Yousry Esam-Eldin; Elsebaey, Mohamed A published the artcile< Sofosbuvir/Ledipasvir for Treatment of Chronic Hepatitis C Infection in Adult Patients with β- Thalassemia Major: A Real-Life Study.>, Reference of 58-97-9, the main research area is HCV; blood diseases; chronic liver disease; cirrhosis; sofosbuvir/ledipasvir; β- Thalassemia major.

BACKGROUND & AIMS: Patients with thalassemia have a lifelong need for blood transfusion, which makes them more risky to hepatitis C virus (HCV). Iron overload and chronic HCV are considered risk factors for patients with thalassemia to develop liver insults. The aim of the present study is to investigate the safety and efficacy of sofosbuvir/ledipasvir in the treatment of chronic HCV infection in Egyptian adult patients with β- thalassemia major. METHODS: A retrospective study included 53 patients with β-thalassemia major with chronic HCV treated with sofosbuvir (400 mg) and ledipasvir (90 mg) as a single pill fixed-dose combination once daily for 12 weeks. The effectiveness of the treatment was assessed by the sustained virologic response (SVR) at 12 weeks after the end of the treatment. RESULTS: SVR was achieved in 96.23% of patients. 47.17% of patients had minor side effects. There was a significant reduction in ALT, AST, and serum ferritin 12 weeks post-therapy. There was an insignificant change in hemoglobin level or blood transfusion requirement 12 weeks posttherapy. There was no change in iron chelators doses throughout the study period. CONCLUSION: Sofosbuvir/ledipasvir regimen seems to be safe and highly effective in the treatment of chronic HCV in patients with β-thalassemia major.

Endocrine, metabolic & immune disorders drug targets published new progress about 58-97-9. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Reference of 58-97-9.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Ito, Yasuhiro; Hirokawa, Mitsuyoshi; Hayashi, Toshitetsu; Kihara, Minoru; Onoda, Naoyoshi; Miya, Akihiro; Miyauchi, Akira published the artcile< Clinical outcomes of follicular tumor of uncertain malignant potential of the thyroid: real-world data.>, HPLC of Formula: 58-97-9, the main research area is Follicular tumor of uncertain malignant potential; Prognosis; Thyroid.

Thyroid tumors arising from follicular cells can generally be divided into malignant and benign tumors. However, some cases are difficult to clearly diagnose whether they are benign or malignant. Therefore, in the most recent version of World Health Organization (WHO) classification, some borderline lesions such as follicular tumor of uncertain malignant potential (FT-UMP), well-differentiated tumor of uncertain malignant potential (WDT-UMP), and noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) were proposed. In this study, we investigated the clinical aspects, including the prognosis, of FT-UMP patients. We investigated the clinical features of 339 patients with FT-UMP. On ultrasound, 68% of the tumors were diagnosed as intermediate, and only 5% of those tumors were diagnosed as malignant. On cytology, 40% of the tumors were diagnosed as follicular neoplasm, and only 1% of these were suspected to be or diagnosed as malignancy. The diagnosis was based on questionable capsular invasion for 332 patients, questionable vascular invasion for 2 patients, and both for 5 patients. Eighty-six percent of the tumors showed low cell proliferation activity. To date, five patients (1%) have shown distant recurrence during postoperative follow-up and underwent various treatments such as radioactive iodine therapy, orthopedic surgery, and denosumab injection. None of these patients have died due to thyroid carcinoma. Our findings suggest that FT-UMP is generally an indolent disease, but some patients show distant recurrence. Physicians should carefully follow patients, although it remains unknown how long they should be observed after surgery.

Endocrine journal published new progress about 58-97-9. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, HPLC of Formula: 58-97-9.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Hodgkinson, Alison; Wall, Clare; Wang, Wendan; Szeto, Ignatius M.; Ye, Wenhui; Day, Li published the artcile< Nucleotides: an updated review of their concentration in breast milk>, Product Details of C9H13N2O9P, the main research area is review nucleotide concentration breast milk; Clinical study; Colostrum; Mature milk; Nucleotides concentrations; Transition milk.

A review. Our knowledge about the complexity of human milk, in particular fatty acid, protein, and oligosaccharide profiles, has increased considerably in recent years. However, little attention has been paid to nucleotides, which account for ∼2% to 5% of the nonprotein nitrogen fraction of breast milk and provide important cellular and metabolism functions for the infant. We examined literature published in the past 25 years to provide an updated review of concentrations of nucleotides in breast milk across lactational stages in mothers around the world. The free mononucleotides found in highest concentrations in breast milk are, from highest to lowest in the order of CMP, UMP, and AMP, GMP, and IMP. Levels of nucleotides varied considerably amongst individual mothers and with stage of lactation. They could be further influenced by time of day and season and the mothers diet. Levels of free nucleotides varied between studies undertaken in different regions; however, in studies that measured total potentially available nucleotides levels, regional differences were not apparent. Some studies report higher amounts in colostrum and transition milk compared with mature milk, whereas other studies report the converse. Recently, clin. studies showed that there are benefits to supplement nucleotides in infant formula. Although comparing data in the literature remains a challenge because of different milk collection methodologies and measurement protocols used by different studies, the information may provide insights for designing of formula products for infant at different stages of development.

Nutrition Research (New York, NY, United States) published new progress about Colostrum. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Product Details of C9H13N2O9P.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Chen, Xiaozhen; Wu, Youjia; Huang, Liying; Yang, Lijuan; Hong, Ruixia; Yao, Hong; Li, Shaoguang published the artcile< Magnetic dispersive solid-phase micro-extraction combined with high-performance liquid chromatography for determining nucleotides in Anoectochilus roxburghii (Wall.) Lindl>, Related Products of 58-97-9, the main research area is magnetic dispersive solid phase microextraction HPLC Anoectochilus nucleotide; Anoectochilus roxburghii (Wall.) Lindl; Fe(3)O(4)@GO nano-adsorbent; High-performance liquid chromatography (HPLC); Magnetic dispersive solid-phase micro-extraction; Nucleotides; Sample pretreatment.

A novel, simple, and efficacious anal. method for determining of nucleotides in Anoectochilus roxburghii (Wall.) Lindl (A. roxburghii) was developed. Magnetic dispersive solid-phase micro-extraction (MDSPME) combined with high-performance liquid chromatog. was applied for extraction and determination of three nucleotides, such as adenosine-5′-monophosphate (AMP), uridine-5′-monophosphate (UMP) and guanosine-5′-monophosphate (GMP) in A. roxburghii from different sources. The structure and morphol. of magnetic nanoparticles, Fe3O4@GO, were illustrated by Fourier-transform IR (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and Thermagravimetric anal. (TGA) techniques. The effects of different extraction conditions on extraction efficiency were investigated and optimized. The optimum extraction conditions were performed as follows: 40.0 mg Fe3O4@GO were dispersed in 30 mL adsorption solution (pH 3.50, 2 μg/mL), 50 mM NaOH was employed for elution with 12 min of ultra-sonication at 40 °C. Under the aforementioned extraction conditions, the Fe3O4@GO nano-adsorbent obtained an excellent adsorption property. The corresponding linearity range for all three analytes exhibited a good linearity (r2 ≥ 0.9982) and notable added recoveries ranging from 88.4% to 109.8%, whereas the limit of quantitation was between 0.8-8 ng/mL. The enrichment factors (EF) were between 174 and 255. The proposed method showed the advantages of full purification, high EF, simplicity, and good recovery. The method was also successfully applied to nucleotides extraction and determination in A. roxburghii, showing superior reproducibility and high sensitivity. Based on this, the method could be expected to provide a novel exptl. means and developmental direction for improving pretreatment and purification of nucleotides, reducing matrix effects as much as possible, in traditional Chinese medicinal materials or biol. specimens.

Journal of Pharmaceutical and Biomedical Analysis published new progress about Adsorption. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Related Products of 58-97-9.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Pan, Calvin Q.; Tiongson, Benjamin C.; Hu, Ke-Qin; Han, Steven-Huy B.; Tong, Myron; Chu, Danny; Park, James; Lee, Tai Ping; Bhamidimarri, Kalyan Ram; Ma, Xiaoli; Xiao, Pei Ying; Mohanty, Smruti R.; Wang, Dan published the artcile< Real-World Study on Sofosbuvir-based Therapies in Asian Americans With Chronic Hepatitis C>, Product Details of C9H13N2O9P, the main research area is sofosbuvir chronic hepatitis C treatment.

Limited data exist with regard to treatment outcomes in Asian Americans with chronic hepatitis C (CHC). We evaluated sofosbuvir (SOF)-based regimens in a national cohort of Asian Americans. Eligible Asian Americans patients with CHC who had posttreatment follow-up of 24 wk for SOF -based therapies from Dec. 2013 to June 2017 were enrolled from 11 sites across the United States. The primary endpoint was sustained virol. response (SVR) rates at posttreatment weeks 12 and 24. Secondary endpoints were to evaluate safety by tolerability and adverse events (AEs). Among 231 patients screened, 186 were enrolled. At baseline, 31% (57/186) patients were cirrhotic, 34% (63/186) were treatment experienced. Most of the subjects (42%, 79/186) received ledispavir/SOF therapy. The overall SVR%, ran12 was 95ging from 86% in genotype (GT) 1b on SOF+ribavirin to 100% in GT 1b patients on ledipasvir/SOF at subgroup analyses. SVR12 was significantly lower in cirrhotic than in noncirrhotic patients [88% (50/57) vs. 98% (126/129), P<0.01]. Stratified by GT, SVR12 were: 96% (43/45) in GT 1a; 93% (67/72) in GT 1b; 100% (23/23) in GT 2; 90% (19/21) in GT 3; 100% (1/1) in GT 4; 83% (5/6) in GT 5; and 100% (16/16) in GT 6. Cirrhotic patients with treatment failure were primarily GT 1, (GT 1a, n = 2; GT 1b, n = 4) with 1 GT 5 (n = 1). Patients tolerated the treatment without serious AEs. Late relapse occurred in 1 patient after achieving SVR12. In Asian Americans with CHC, SOF-based regimens were well tolerated without serious AEs and could achieve high SVR12 regardless of hepatitis C viral infection GT. Journal of Clinical Gastroenterology published new progress about Chronic hepatitis C. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Product Details of C9H13N2O9P.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Gupta, Mrityunjay; Levine, Samantha R.; Spitale, Robert C. published the artcile< Probing Nascent RNA with Metabolic Incorporation of Modified Nucleosides>, HPLC of Formula: 58-97-9, the main research area is .

Conspectus: The discovery of previously unknown functional roles of RNA in biol. systems has led to increased interest in revealing novel RNA mols. as therapeutic targets and the development of tools to better understand the role of RNA in cells. RNA metabolic labeling broadens the scope of studying RNA by incorporating of unnatural nucleobases and nucleosides with bioorthogonal handles that can be utilized for chem. modification of newly synthesized cellular RNA. Such labeling of RNA provides access to applications including measurement of the rates of synthesis and decay of RNA, cellular imaging for RNA localization, and selective enrichment of nascent RNA from the total RNA pool. Several unnatural nucleosides and nucleobases have been shown to be incorporated into RNA by endogenous RNA synthesis machinery of the cells. RNA metabolic labeling can also be performed in a cell-specific manner, where only cells expressing an essential enzyme incorporate the unnatural nucleobase into their RNA. Although several discoveries have been enabled by the current RNA metabolic labeling methods, some key challenges still exist: (i) toxicity of unnatural analogs, (ii) lack of RNA-compatible conjugation chemistries, and (iii) background incorporation of modified analogs in cell-specific RNA metabolic labeling. In this Account, we showcase work done in our laboratory to overcome these challenges faced by RNA metabolic labeling. To begin, we discuss the cellular pathways that have been utilized to perform RNA metabolic labeling and study the interaction between nucleosides and nucleoside kinases. Then we discuss the use of vinyl nucleosides for metabolic labeling and demonstrate the low toxicity of 5-vinyluridine (5-VUrd) compared to other widely used nucleosides. Next, we discuss cell-specific RNA metabolic labeling with unnatural nucleobases, which requires the expression of a specific phosphoribosyl transferase (PRT) enzyme for incorporation of the nucleobase into RNA. In the course of this work, we discovered the enzyme uridine monophosphate synthase (UMPS), which is responsible for nonspecific labeling with modified uracil nucleobases. We were able to overcome this background labeling by discovering a mutant uracil PRT (UPRT) that demonstrates highly specific RNA metabolic labeling with 5-vinyluracil (5-VU). Furthermore, we discuss the optimization of inverse-electron-demand Diels-Alder (IEDDA) reactions for performing chem. modification of vinyl nucleosides to achieve covalent conjugation of RNA without transcript degradation Finally, we highlight our latest endeavor: the development of mutually orthogonal chem. reactions for selective labeling of 5-VUrd and 2-vinyladenosine (2-VAdo), which allows for potential use of multiple vinyl nucleosides for simultaneous investigation of multiple cellular processes involving RNA. We hope that our methods and discoveries encourage scientists studying biol. systems to include RNA metabolic labeling in their toolkit for studying RNA and its role in biol. systems.

Accounts of Chemical Research published new progress about 58-97-9. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, HPLC of Formula: 58-97-9.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Morgado-Carrasco, Daniel; Combalia, Andrea; Fustà-Novell, Xavier; Mascaró, José Manuel Jr; Iranzo, Pilar published the artcile< Aggressive erosive lichen planus associated with hepatitis C responding to sofosbuvir/ledipasvir treatment.>, Synthetic Route of 58-97-9, the main research area is .

There is no abstract available for this document.

Indian journal of dermatology, venereology and leprology published new progress about 58-97-9. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Synthetic Route of 58-97-9.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Mineguchi, Yuri; Goto, Kyosuke; Sudo, Yuna; Hirayama, Kentaro; Kashiwagi, Hirotoshi; Sasagase, Izumi; Kitazawa, Haruki; Asakuma, Sadaki; Fukuda, Kenji; Urashima, Tadasu published the artcile< Characterisation of sugar nucleotides in colostrum of dairy domestic farms animals>, Recommanded Product: ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate, the main research area is colostrum sugar nucleotide UMP UDP.

The biol. significance and heterogeneity of bovine, caprine and ovine colostrum sugar nucleotides is unclear. Colostrum sugar nucleotides and sugar monophosphates were purified and characterised with 1H-NMR spectroscopy. UDP-galactose (UDP-Gal) and UDP-glucose (UDP-Glc) were identified in colostrum of Holstein and Brown Swiss cows; UDP-Gal, UDP-Glc, UDP-N-acetylgalactosamine (UDP-GalNAc) and UDP-N-acetylglucosamine (UDP-GlcNAc) were found in Japanese Saanen goat colostrum. UDP-Gal, UDP-Glc, UDP-GalNAc and UDP-GlcNAc were found in Corriedale sheep but not in the Suffolk and Fraise Land breeds. The presence/absence of these sugar nucleotides in colostrum is heterogeneous among dairy domestic farm animals and the three sheep breeds. In addition, a nucleotide, uridine-ribose diphosphate (UDP) was identified in the colostra of Japanese Saanen goat, and Suffolk and Fraiseland sheep, while uridine-ribose monophosphate (UMP) was detected in the colostrum of Brown Swiss cow and the three breed sheep. N-acetylglucosamine-1-phosphate (GlcNAc-1-P) was also identified in the colostrum of the goat and two cow breeds.

International Dairy Journal published new progress about Bos taurus. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Recommanded Product: ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate.

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem