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Comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC × GC-TOF-MS) was combined with conventional mono-dimensional GC?MS to differentiate Croatian virgin olive oils (VOO) according to variety and geographical origin, based on the profile of volatile aroma compounds isolated by HS-SPME. More than 1000 compounds were detected after untargeted profiling and 256 were identified or tentatively identified, providing one of the most detailed profiles of volatile aroma compounds in VOO up to date. Among them, 131 volatile compounds were significantly different across monovarietal VOOs, while 60 were found useful for the discrimination according to geographical origin. Many major lipoxygenase and minor non-lipoxygenase-generated compounds were shown to have discriminating ability with respect to both factors. Multivariate statistical analysis extracted twenty-one volatile markers with the highest discriminant power for varietal differentiation. The approach reported may have practical application in better understanding, defining, managing, and communicating the varietal or geographical typicity of monovarietal VOOs.

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Tetrahydrofuran – Wikipedia,
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There is a growing market for the use of hydrolysates from animal side-streams for production of high-protein supplements. However, there can be issues with development of off-flavors, either due to the raw material in question or due to the hydrolysis process itself. This study examined the development of volatile compounds during hydrolysis of hemoglobin. Briefly, porcine hemoglobin was hydrolyzed by 0.5% papain for up to 5 h, and the development of volatile compounds was analyzed via gas chromatography-mass spectrometry. The results showed that there was significant development of a number of volatile compounds with time, e.g., certain Maillard reaction and lipid oxidation products, which are likely candidates for the aroma development during hydrolysis. Furthermore, it was shown that development of a number of the volatiles was due to the hydrolysis process, as these compounds were not found in a control without enzyme.

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Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

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Poplar (Populus nigra) flash pyrolysis has been performed at the IK4-Ikerlan 25 kg h-1 pilot plant equipped with a conical spouted bed reactor. Gas, bio-oil and char yields and properties have been studied in the 425-525 C range. This reactor has been proven to be especially suitable for this process as high bio-oil yields have been obtained, with the maximum being 69 wt.% at 455 C. The bio-oil has been collected in two fractions: the lighter one, which accounts for 85 wt.% of the bio-oil, has a high water content and is composed mainly of acids and ketones, whereas the heavier fraction has a lower water content and is rich in phenols. These fractions are miscible, obtaining a bio-oil with a water content lower than 25 wt.% and a higher heating value in the 16-18 MJ kg-1 range.

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Tetrahydrofuran – Wikipedia,
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A simultaneous multiple solid-phase microextraction-single shot-gas chromatography mass spectrometry (smSPME-ss-GC/MS) method has been developed for headspace analysis. Up to four fibers (50/30mum DVB/CAR/PDMS) were used simultaneously for the extraction of aroma components from the headspace of a single sample chamber in order to increase sensitivity of aroma extraction. To avoid peak broadening and to maximize resolution, a simple cryofocusing technique was adopted during sequential thermal desorption of multiple SPME fibers prior to a ‘single shot’ chromatographic run. The method was developed and validated on a model flavor mixture, containing 81 known pure components. With the conditions of 10min of incubation and 30min of extraction at 50C, single, dual, triple and quadruple SPME extractions were compared. The increase in total peak area with increase in the number of fibers showed good linearity (R2=0.9917) and the mean precision was 12.0% (RSD) for the total peak sum, with quadruple simultaneous SPME extraction. Using a real sample such as commercial coffee granules, aroma profile analysis was conducted using single, dual, triple and quadruple SPME fibers. The increase in total peak intensity again showed good linearity with increase in the number of SPME fibers used (R2=0.9992) and the precision of quadruple SPME extraction was 9.9% (RSD) for the total peak sum.

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Optimised extraction methods are required to better understand the impact of volatile compounds on the physical and organoleptic attributes of baked confectionary products (cakes, etc.). This is especially relevant with an increased focus on the reformulation of such products to aid in the reduction of diet-related chronic diseases. Headspace solid-phase microextraction (HS-SPME) has become one of the most widely used extraction techniques for volatile profiling of foods and beverages, mainly because it is very automatable, has a high sample throughput, is solvent-free and multiple fibre phases are available to target a wide range of volatile organic compounds. This study used response surface methodology to optimise HS-SPME parameters for the extraction of volatiles in baked confectionary products. After HS-SPME fibre selection, a central composite design was used to evaluate the effect of incubation time, extraction time and extraction temperature on 18 selected volatile compounds, representative of key volatiles in baked confectionary products, using a sponge cake crumb as the matrix. The most suitable fibre was the divinylbenzene/carboxen/polydimethylsiloxane. The results demonstrated that the final reduced models significantly (p < 0.0001) fitted the responses of 18 selected volatile compounds, with R2 values ranging from 0.8178 to 0.9871. The optimal conditions derived were an incubation time of 5 min, extraction time of 60 min and an extraction temperature of 60 C. These were subsequently evaluated in three baked confectionary products, highlighting the effectiveness of this approach. I hope this article can help some friends in scientific research. I am very proud of our efforts over the past few months and hope to 105-21-5, help many people in the next few years.Recommanded Product: Gamma-heptalactone

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Tetrahydrofuran – Wikipedia,
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Flavour plays a crucial role in food and is the most important aspect of milk. Milk has a pleasant smell and milk flavour can be used in several products. However, how to recreate milk flavour has rarely been reported. Therefore, the creation of milk flavour is of great interest. This study focused on the creation and imitation of milk flavour. The approach to flavour creation involves identifying the attributes of the flavour and developing an impactful, authentic flavour. Accordingly, to create a milk flavour, the notes of milk flavour were identified by smelling and tasting milk. Based on the identified notes, aroma ingredients were selected to blend into a milk-flavour. After olfactory discrimination, several modifications and adjustments were made and a typical milk flavour formula was obtained. The results showed that the milk flavour has the following notes: milky, acidic, vanilla-like, caramel-like, aldehydic, fruity, beany, buttery, meaty, and vegetative. Lactones, acids, aldehydes, alcohols, ketones, esters, sulphur compounds, furans, and nitrogen compounds can provide these notes. Based on the developed formula, a harmonious flavour with typical characteristics of milk was obtained by blending the selected aroma ingredients.

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Tetrahydrofuran – Wikipedia,
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The aim of this study was to investigate the aroma and sensory profiles of various types of peaches (Prunus persica L. Batsch.). Forty-three commercial cultivars comprising peaches, flat peaches, nectarines, and canning peaches (pavias) were grown over two consecutive harvest years. Fruits were assessed for chemical aroma and sensory profiles. Chemical aroma profile was obtained by proton transfer reaction-mass spectrometry (PTR-MS) and spectral masses were tentatively identified with PTR-Time of Flight-MS (PTR-Tof-MS). Sensory analysis was performed at commercial maturity considering seven aroma/flavor attributes. The four types of peaches showed both distinct chemical aroma and sensory profiles. Flat peaches and canning peaches showed most distinct patterns according to discriminant analysis. The sensory data were related to the volatile compounds by partial least square regression. gamma-Hexalactone, gamma-octalactone, hotrienol, acetic acid and ethyl acetate correlated positively, and benzeneacetaldehyde, trimethylbenzene and acetaldehyde negatively to the intensities of aroma and ripe fruit sensory scores.

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This study aimed to assess the effect of fermentation time and potato pulp addition on the fermentation characteristics of dough and textural profiles of the steamed bread. Volatile compounds in steamed bread processing were also determined. Rheofermentometer analysis showed that the gas retention capability of dough decreased with the increase in potato pulp level, and the productive fermentation time might be shortened through the addition of high potato pulp levels (40%?50%) to dough. The resistance to extension of dough samples decreased with the increase in fermentation time and potato pulp level. When the fermentation time was 60?75 min, all steamed bread samples had high specific volumes and soft textures. Moreover, the volatile compounds in the fermented dough and steamed bread were enriched by the addition of potato pulp. These results indicated that fermentation time and potato pulp affected the technological properties and volatile compounds of wheat dough and steamed bread.

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Tetrahydrofuran – Wikipedia,
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Cold storage is used to delay the senescence of peaches, but it can also lead to internal browning and aroma loss. Modified atmosphere packaging (MAP) has been reported to inhibit the internal browning and prolong the storage time. Four MAP treatments in the present study were set as follows: I: O2 1% to 3%, CO2 3% to 5%, and N2 92% to 96%; II: O2 3% to 5%, CO2 3% to 5%, and N2 90% to 94%; III: O2 6% to 8%, CO2 3% to 5%, and N2 87% to 91%; and control (CK): O2 21%, CO2 0.03%, and N2 79%. The concentration of sugars, acids, aroma compounds, superoxide radical (O2?), hydrogen peroxide (H2O2), and malondialdehyde (MDA), as well as the activities of enzymes, such as superoxide dismutase (SOD), peroxidase (POD), lipoxygenase (LOX), hydroperoxide lyase (HPL), alcohol dehydrogenase, and alcohol O-acyltransferase (AAT) activities, were investigated. The results revealed that MAP, especially for treatment II, could inhibit the loss of flavors such as sugars, acids, and aroma compounds; maintain higher SOD and POD activities; and inhibit the accumulation of O2?, H2O2, and MDA during shelf life after storage at low temperature for 30 days. It could also inhibit the LOX and HPL activities at low temperature, but maintain higher LOX and HPL activities during shelf life. These findings indicated that treatment II could prolong the storage time to 30 days and shelf life for 3 days; maintain the higher content of sugars, acids, and aroma compounds; protect the cell membrane from oxidative injury; and inhibit internal browning during cold storage and shelf life.

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Tetrahydrofuran – Wikipedia,
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Agricultural manipulation of potentially beneficial rhizosphere microbes is increasing rapidly due to their multi-functional plant-protective and growth related benefits. Plant growth promoting rhizobacteria (PGPR) are mostly non-pathogenic microbes which exert direct benefits on plants while there are rhizosphere bacteria which indirectly help plant by ameliorating the biotic and/or abiotic stress or induction of defense response in plant. Regulation of these direct or indirect effect takes place via highly specialized communication system induced at multiple levels of interaction i.e., inter-species, intra-species, and inter-kingdom. Studies have provided insights into the functioning of signaling molecules involved in communication and induction of defense responses. Activation of host immune responses upon bacterial infection or rhizobacteria perception requires comprehensive and precise gene expression reprogramming and communication between hosts and microbes. Majority of studies have focused on signaling of host pattern recognition receptors (PRR) and nod-like receptor (NLR) and microbial effector proteins under mining the role of other components such as mitogen activated protein kinase (MAPK), microRNA, histone deacytylases. The later ones are important regulators of gene expression reprogramming in plant immune responses, pathogen virulence and communications in plant-microbe interactions. During the past decade, inoculation of PGPR has emerged as potential strategy to induce biotic and abiotic stress tolerance in plants; hence, it is imperative to expose the basis of these interactions. This review discusses microbes and plants derived signaling molecules for their communication, regulatory and signaling networks of PGPR and their different products that are involved in inducing resistance and tolerance in plants against environmental stresses and the effect of defense signaling on root microbiome. We expect that it will lead to the development and exploitation of beneficial microbes as source of crop biofertilizers in climate changing scenario enabling more sustainable agriculture.

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Reference:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem