Microbial enzyme helps to churn out scarce COVID drug at full tilt was written by Anonymous. And the article was included in Nature (London, United Kingdom) in 2022.Name: ((2R,3S,4R,5R)-3,4-Dihydroxy-5-((Z)-4-(hydroxyimino)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl isobutyrate The following contents are mentioned in the article:
An engineered bacterial enzyme turbocharges the manufacture of the antiviral molnupiravir, one of the few approved drugs that can prevent severe COVID-19. Current methods for making the drug require multiple steps and yield relatively low amounts A more efficient route involves a precursor mol. called N-hydroxycytidine: just one chem. reaction converts it to molnupiravir. Anthony Green at the University of Manchester, UK, and his colleagues observed that, under certain conditions, an enzyme from the bacterium Escherichia coli could make small amounts of the precursor from the biomol. cytidine (J. Am. Chem. Soc. 144, 3761-3765 (2022)). The team mutated the DNA encoding the enzyme, improving the enzyme’s efficiency, and used E. coli cells to make the upgraded enzyme. Finally, the researchers optimized the synthetic process by lowering the reaction’s temperature That prompted N-hydroxycytidine to crystallize in high purity from solution as soon as it formed. In a scaled-up demonstration, the authors prepared 137 g of N-hydroxycytidine, converting 71% of reactants to the precursor mol. in under 3 h. During that time, each enzyme mol. facilitated more than 85,000 chem. transformations. This study involved multiple reactions and reactants, such as ((2R,3S,4R,5R)-3,4-Dihydroxy-5-((Z)-4-(hydroxyimino)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl isobutyrate (cas: 2492423-29-5Name: ((2R,3S,4R,5R)-3,4-Dihydroxy-5-((Z)-4-(hydroxyimino)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl isobutyrate).
((2R,3S,4R,5R)-3,4-Dihydroxy-5-((Z)-4-(hydroxyimino)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl isobutyrate (cas: 2492423-29-5) belongs to tetrahydrofuran derivatives. Tetrahydrofurans and furans are important oxygen-containing heterocycles that often exhibit interesting properties for biological applications or applications in the cosmetic industry. It is more basic than diethyl ether and forms stronger complexes with Li+, Mg2+, and boranes. It is a popular solvent for hydroboration reactions and for organometallic compounds such as organolithium and Grignard reagents.Name: ((2R,3S,4R,5R)-3,4-Dihydroxy-5-((Z)-4-(hydroxyimino)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl isobutyrate
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem