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A combination of pyrolysis-GC/MS and TLC-FID techniques were used for whole sample analysis of bio-oil samples obtained from different liginocellulosic biomasses, (i.e., birch wood, pine wood, barley straw and forest residue and thermal-cracking fractions). Both techniques showed the ability to analyze the whole sample without cleanup or fractionation and to distinguish among the bio-oils based on their feedstock sources. The TLC-FID is a chromatographic method whereby the oil solution is spotted on a TLC rod, developed by a two solvent system and the chromatographic bands measured by flame ionization detection (FID). The Py-GC/MS instrument uses a sample cup and a vertical microfurnace to analysis bio-oils. The non-volatiles fraction remaining in the cup were weighed after thermal evaporation-GC/MS. The hardwood birch bio-oil was shown to have syringol and its derivatives compared to pine wood and barley straw bio-oils that were shown to have methoxyphenolic and non-methoxy-phenolic compounds, respectively. TLC-FID shows clear differences in the peak areas and shapes for the bio-oils fractions by solvent development on chromarods and are in agreement with the results obtained by Py-GC/MS. Furthermore, forest residue bio-oil and its thermal cracking fractions could be effectively characterized by TLC-FID (and Py-GC/MS) whereby the light fraction was composed of a wide range of lower polarity compounds while middle and heavy fraction had higher polarity compounds.
Each elementary reaction can be described in terms of its molecularity, the number of molecules that collide in that step. The slowest step in a reaction mechanism is the rate-determining step.you can also check out more blogs about 4100-80-5. 4100-80-5
Reference：
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem